RNA interference : key applications

Gene knockdown by RNA interference (RNAi) is the best and most cost-effective way to study genotype-phenotype correlations and protein functions in mammalian cells. It is fast, reliable and potent. Traditional RNAi reagents for gene knockdown consist of double stranded RNA annealed together in a RNA duplex with 3´-overhangs. RNAi reagents used include small interferring RNA (siRNA) and miRNA mimics.

riboxx has developped and validated in more than 75 publications a new technology called the RNAi-cap® for RNAi molecules.

The RNAi-cap improves knockdown potency, reduces off target effects and enhances dramatically stability of the RNA duplex. As a consequence, less siRNA is needed for potent and long lasting gene knockdown.

The RNAi-cap is included in all siRNA and miRNA mimics of Riboxx, particularly the iBONi® siRNA for gene knockdown in cell culture, the iVORi® siRNA for in VIVO gene knockdown and the CONmiR® miRNA mimics.

riboxx offers a broad range of RNAi reagents for in vitro and in vivo gene knockdown. Your choice is mainly determined by experimental parameters (cell culture or in vivo experiment) and the type of RNAi reagent needed (siRNA or miRNA).

 

Applications of RNA interference

  • gene knockdown in cell culture
  • control of gene knockdown
  • gene knockdown with miRNA
  • gene knockdown in VIVO
  • RNAi transfection

 

RNA interference : which reagent do I need?

riboxx offers a broad range of RNAi reagents for in vitro and in vivo gene knockdown. Your choice is mainly determined by experimental parameters (cell culture or in vivo experiment) and the type of RNAi reagent needed (siRNA or miRNA), as shown below.

         
  gene knockdown
in cell culture
gene knockdown
in VIVO
miRNA mimic
in cell culture
miRNA mimic
in VIVO
recommended
products
 

iBONi siRNA

iVORi siRNA
CONmiR mimic CONmiR in VIVO

potency
(recomm. conc.)

25 nM per well
2-4 mg/kg per application
25 nM per well
2-4 mg/kg per application
guarantee 2 of 3 siRNA guaranteed HPLC, 2x desalted,
endotoxin free
miRNA mimic guaranteed HPLC, 2x desalted,
endotoxin free
off-target effects lowest lowest lowest lowest
specificty highest highest highest highest
molecular structure blunt dsRNA, 24 bp,
with RNAi-cap
blunt dsRNA, 24 bp,
with RNAi-cap
blunt dsRNA, 24 bp,
with RNAi-cap
blunt dsRNA, 24 bp,
with RNAi-cap
target species
human, rat, mouse
 human, rat, mouse
 human, rat, mouse  human, rat, mouse
coverage  coding & non-coding RNA
 coding  RNA  coding & non-coding RNA  coding  RNA

 

riboxx RNAi reagents : overview

riboxx offers a broad range of RNAi reagents for in vitro and in vivo gene knockdown, including HT libraries and custom siRNA and miRNA mimics with chemical modifications, cholesterol labels or fluorophores, among others. A comprehensive overview of the reagents is shown below.

 custom siRNA 

1 x siRNA (2 nmol, 5 nmol or 10 nmol) iBONi siRNA one
 pre-designed siRNA 
  1 x siRNA + 1 x neg. ctrl. (2 or 5 nmol each) iBONi siRNA plus  
 
3 x siRNA + 1 x neg. ctrl. (2 or 5 nmol each) iBONi siRNA quattro
  3 x pooled siRNA (Total amount: 5 or 10 nmol) iBONi siRNA pool  
 pre-designed siRNAs + transfection reagent + controls
  3 x siRNAs + 1 x neg. ctrl. (2 or 5 nmol each) + transfection reagent
iBONi siRNA box  
 siRNA controls
  siRNA negative controls (2 or 5 nmol) iBONi siRNA negative control  
  siRNA positive controls (2 or 5 nmol) iBONi siRNA positive control  
  siRNA transfection controls (2 nmol) iBONi siRNA transfection control  
 siRNA for in VIVO gene knockdown
  1 siRNA HPLC, desalted, endotoxin free (20, 100, 200 or 500 nmol) iVORi siRNA in VIVO  
 miRNA mimics
  1 x miRNA mimic (2, 5 nmol or 10 nmol) CONmiR mimic  
  1 x miRNA mimic (2 or 5 nmol) + 1 neg. control CONmiR mimic plus  
  1 x miRNA mimic (2 or 5 nmol) + 1 neg. control + transfection reagent CONmiR mimic box  
  1 x miRNA mimic HPLC, desalted, endotoxin free (20, 100, 200 or 500 nmol) CONmiR mimic in VIVO  
 miRNA inhibitor
  1 x miRNA mimic (5 nmol or 10 nmol) CONTRAmiR inhibitor  
  1 x miRNA inhibitor HPLC, desalted, endotoxin free (20, 100, 200  or 500 nmol) CONTRAmiR inhibitor in VIVO  
controls for miRNA mimic & miRNA inhibitors
  miRNA mimic negative control (2 and 5 nmol) CONmiR negative control  
  miRNA inhibitor negative control (2 and 5 nmol) CONTRAmiR negative control  

 

 

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