1 x potent RIG-I ligand for activation of immune cells in vivo
Characteristics: dsRNA duplex with 5´-ppp, 20 bp in length
Amount: 500 µg or 1 mg
Base composition: cytosines, guanosines
Purification: RP/IEX, HPLC
Quality Control: PAGE, desalted, endotoxin free
RIGOXXON CLINIgrade is a synthetic double stranded RNA (dsRNA) with a 5´-triphoshate (5´-ppp). It has a length of 20 bp. It is composed of cytosines and guanosines.
Double stranded RNA with a 5´-ppp is a potent activator of innate immunity. In the context of innate immunity, 5´-ppp-dsRNA is a pathogen associated molecular pattern (PAMP) that activates innate immune response through pathogen recognition receptors (PRR). The PRR of 5´-ppp-dsRNA is the retinoic-acid inducible gene I (RIG-I) protein [1,2].
RIG-I is present in the cytosol of antigen presenting cells such as dendritic cells (DCs) and monocytes . Signaling of RIG-I is triggered by a dsRNA bearing a 5´-triphosphate (5´-ppp) .
Triggering the RIG-I-pathway IFN-α and IFN-β production, improves cross-presentation of antigens and MHC class I expression.
In animal models, 5´-ppp-dsRNA enhances the specific T cell responses. Moreover, RIGOXXON CLINIgrade displays a resistance to serum and body fluids. It is therefore ideal for experimental set-up in cell culture supplemented with Fetal Calf Serum (FCS) and in small animal models.
1. Schmidt a. et al., 2009. 5'- triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I. PNAS 106(29):12067-72.
2. Yoneyama M. & Fujita T., 2007. Function of RIG-I-like Receptors in Antiviral Innate Immunity. J. Biol. Chem. 282: 15315-8.
3. Hornung V. et al., 2006. 5'-Triphosphate RNA is the ligand for RIG-I. Science. 314(5801):994-7.
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