RIGOXXON negative control
1x dsRNA of 20 bp without 5´-ppp as a negative control for RIGOXXON
Characteristics: dsRNA duplex without 5´-ppp, 20 bp in length
Amount: 500 µg or 1 mg
Base composition: cytosines, guanosines
Purification: RP/IEX, HPLC
Quality Control: PAGE
RIGOXXON negative control is a synthetic double stranded RNA (dsRNA) without 5´-triphoshate (5´-ppp). It has a length of 20 bp. It is composed of cytosines and guanosines.
Double stranded RNA with a 5´-ppp is a potent activator of innate immunity. In the context of innate immunity, 5´-ppp-dsRNA is a pathogen associated molecular pattern (PAMP) that activates innate immune response through pathogen recognition receptors (PRR). The PRR of 5´-ppp-dsRNA is the retinoic-acid inducible gene I (RIG-I) protein [1,2].
RIG-I is present in the cytosol of antigen presenting cells such as dendritic cells (DCs) and monocytes . Signaling of RIG-I is triggered by a dsRNA bearing a 5´-triphosphate (5´-ppp) .
Triggering the RIG-I-pathway IFN-α and IFN-β production, improves cross-presentation of antigens and MHC class I expression.
RIGOXXON negative control does not display the 5´-ppp and hence does not allow activation of immune cells through RIG-I ligation.
1. Schmidt a. et al., 2009. 5'- triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I. PNAS 106(29):12067-72.
2. Yoneyama M. & Fujita T., 2007. Function of RIG-I-like Receptors in Antiviral Innate Immunity. J. Biol. Chem. 282: 15315-8.
3. Hornung V. et al., 2006. 5'-Triphosphate RNA is the ligand for RIG-I. Science. 314(5801):994-7.
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