riboLINK miRNA biotin

€283.00
SKU
L-00210

To order riboLINK miRNA biotin, please enter the sequence of mature miRNA
(5p- or 3p-, 19 - 25 nt in length, valid inputs are: 'G'; 'C'; 'A'; 'U'; 'g'; 'c'; 'a'; 'u')

Maximum number of characters: 25

1x pre-designed miRNA mimic linked to a biotin with an endosome cleavable linker

Scale: 2, 5 or 10 nmol
Functional group: biotin
Purification: RP/PS
Quality control:
PAGE
Delivery: dried

riboLINK miRNA allows reversible coupling of miRNA mimic to a functional group such as a fluorophore or biotin. The linker of riboLINK miRNA is pH-sensitive. After uptake and internalization of riboLINK miRNA to the endosome, the linker is cleaved in the acidic milieu of the late endosome, releasing the siRNA that displays its full biological activity.

Moreover, riboLINK miRNA has the RNAi-cap technology.
The RNAi-cap technology offers several advantages:

  • directional modulation of RISC unwinding of miRNA duplex, enhancing loading of the guide strand to the RISC complex. As a consequence, much less miRNA are needed for an effective gene silencing with a subsequent reduction of off-target effects [1]
  • improved stability of miRNA duplex with subsequent improved half-life in body fluids.

References

[1] Nolte A, Ott K, Rohayem J, Walker T, Schlensak C, Wendel HP. Modification of small interfering RNAs to prevent off-target effects by the sense strand. N Biotechnol. 2013 Jan 25;30(2):159-65.

More Information
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Application notes

Most recent citations

Parsi S et al. Mol Ther Nucleic Acids. 2015 Oct 6.
Smith PY et al. Hum Mol Genet. 2015 Dec 1.
Dusl M et al. Hum Mol Genet. 2015 Mar 12.
Porstner M et al. Eur J Immunol. 2015 Apr.
Tatikotta SG. Freie Universität Berlin, 2015.

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