iBONi siRNA positive control-P4M

iBONi siRNA controls are designed to be used as positive, negative or transfection controls for validation of gene silencing experiments. iBONi siRNA controls originate either from published experimental data and have been successfully tested by independent scientists in human, mouse and rat cells (please refer to citations of our customers).
All iBONi siRNA controls include the RNAi-cap. The RNAi-cap is located at the opposite position of the seed-region.

The RNAi-cap technology offers several advantages:

• directional modulation of RISC unwinding of siRNA duplex, enhancing loading of the guide strand to the RISC complex. As a consequence, much less siRNA is needed for an effective gene silencing with a subsequent reduction of off-target effects [1]
• improved stability of siRNA duplex with subsequent improved half-life in body fluids.

References
[1] Nolte A, Ott K, Rohayem J, Walker T, Schlensak C, Wendel HP. Modification of small interfering RNAs to prevent off-target effects by the sense strand. N Biotechnol. 2013 Jan 25;30(2):159-65.