iBONi siRNA negative control-N2

€98.00
SKU
K-00601

1 x non-targeting siRNA to control gene knockdown specificity

Amount:
2 or 5 nmol
Purification: RP-HPLC
Quality control: PAGE
Delivery: dried

iBONi siRNA controls are designed to be used as positive, negative or transfection controls for validation of gene silencing experiments. iBONi siRNA controls originate either from published experimental data and have been successfully tested by independent scientists in human, mouse and rat cells (please refer to citations of our customers).
All iBONi siRNA controls include the RNAi-cap. The RNAi-cap is located at the opposite position of the seed-region.

The RNAi-cap technology offers several advantages:
• directional modulation of RISC unwinding of siRNA duplex, enhancing loading of the guide strand to the RISC complex. As a consequence, much less siRNA is needed for an effective gene silencing with a subsequent reduction of off-target effects [1]
• improved stability of siRNA duplex with subsequent improved half-life in body fluids.

References
[1] Nolte A, Ott K, Rohayem J, Walker T, Schlensak C, Wendel HP. Modification of small interfering RNAs to prevent off-target effects by the sense strand. N Biotechnol. 2013 Jan 25;30(2):159-65.

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iBONi siRNA controls

Application notes

Most recent citations

Saalbach A et al. Exp Dermatol. 2015 Apr 11.
Krossa S et al. Oncotarget. 2015 Apr 18.
Schomaker M et al. J Nanobiotechnology. 2015 Feb 3;13(1):10.
Troiano A et al. J Cell Physiol. 2015 Jan 29.
Knoch KP et al. Mol Metab. 2014 May 14;3(5):518-30.
Anwar SL et al. PLoS Pathog 2012 Nov 7;11.
Scudieri P et al. J. Physiol. 2012 Dec; 590(23):6141–6155.
Willenberg A et al. J Invest Dermatol. 2012 Feb;132(2):385-93.

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