CONmiR negative control-N2

€124.00
SKU
K-01010

1 x non-targeting CONmiR mimic to control gene knockdown specificity

Amount:
2 or 5 nmol
Purification: RP/HPLC
Quality control: PAGE
Delivery: dried

CONmiR controls are designed to be used as positive or negative controls for validation of gene knockdown experiments. CONmiR controls originate either from published experimental data or have been successfully tested by independent scientists in human, mouse and rat cells (please refer to citations of our customers).
All CONmiR controls include the RNAi-cap for miRNA mimics. The RNAi-cap is located at the opposite position of the seed-region.

The RNAi-cap technology offers several advantages:

  • directional modulation of RISC unwinding of miRNA duplex, enhancing loading of the guide strand to the RISC complex. As a consequence, much less miRNA is needed for an effective gene knockdown with a subsequent reduction of off-target effects [1]
  • improved stability of the miRNA duplex with subsequent improved half-life in body fluids.

References
[1] Nolte A, Ott K, Rohayem J, Walker T, Schlensak C, Wendel HP. Modification of small interfering RNAs to prevent off-target effects by the sense strand. N Biotechnol. 2013 Jan 25;30(2):159-65.

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CONmiR miRNA mimics with gene knockdown bonus

Application notes

Most recent citations

Parsi S et al. Mol Ther Nucleic Acids. 2015 Oct 6.
Smith PY et al. Hum Mol Genet. 2015 Dec 1.
Dusl M et al. Hum Mol Genet. 2015 Mar 12.
Porstner M et al. Eur J Immunol. 2015 Apr.
Tatikotta SG. Freie Universität Berlin, 2015.

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